Sphingomonas mucosissima Bacteremia in Patient with Sickle Cell Disease

To the Editor: The genus Sphingomonas was proposed by Yabuuchi et al. in 1990 (1) and amended by Takeuchi et al. in 1993 (2). It now has been subdivided into 4 separate genera: Sphingomonas sensu stricto, Sphingobium, Novosphingobium, and Sphingopyxis. The bacteria of the genus Sphingomonas are yellow-pigmented, nonfermenting, gramnegative bacilli with a single polar fl agellum; they are widely distributed in the natural environment, especially in water and soil (3). These bacteria are characterized by the presence of a unique sphingoglycolipid with the long-chain base—dihydrosphingosin, ubiquinone 10 (Q-10), and 2-hydroxymyristic acid (2-OH C14:0)—and the absence of 3-hydroxy fatty acids (4). S. mucosissima was isolated and identifi ed in 2007 by Reddy and Garcia-Pichel from biologic soil crust samples collected from sandy arid soil in the US Colorado Plateau (5). Sphingomonas spp. are opportunistic pathogens and have recently been implicated in a variety of communityacquired and nosocomial infections, considered to originate from contaminated hospital equipment or manipulation of some medical devices (3). The survival of Sphingomonas spp. in indoor dust particles as aerosols and their resistance to many disinfecting and toxic chemicals may explain their ability to colonize medical devices such as mechanical ventilators, catheters, and bronchofi beroscopes (6). In the past few years, these organisms, in particular S. paucimobilis, have been implicated in a variety of communityacquired and nosocomial infections. We report a case of S. mucosissima bacteremia in a patient with sickle cell disease. In February 2008, a 17-yearold woman with homozygous sickle cell anemia was hospitalized when her condition suddenly became worse. The patient had undergone a splenectomy in 1992 and a cholecystectomy in February 2007. Four days after admission, she had a fever of 38.7°C. Two aerobic blood specimens, drawn on the fi fth day of her hospitalization, yielded gram-negative bacilli after a 24-hour incubation. The gram-negative bacilli were positive for catalase and oxidase but remained unidentifi ed by API 20NE strip (bioMérieux, Marcy l’Etoile, France). MICs of antimicrobial drugs were determined for the gram-negative bacilli by using an Etest assay (AB BIODISK, Solna, Sweden) on Mueller-Hinton medium. MICs were 1 μg/mL for cefotaxime, 1 μg/mL for amoxicillin–clavulanic acid, 2–3 μg/mL for vancomycin, 0.064 μg/mL for imipenem, 4–5 μg/mL for ceftazidime, 1 μg/mL for amikacin, 3 μg/mL for ciprofl oxacin, and 0.047 μg/mL for trimethoprim-sulfamethoxazole. DNA was extracted from 1 colony by using a QIAamp Tissue kit (QIAGEN, Hilden, Germany) as described by the manufacturer. A 16S rDNA sequence was obtained (1,410 bp) by using the fD1 (5′AGAGTTTGATCCTGGCTCAG-3′) and rP2 (5′-ACGGCTACCTTGTTAC GACTT-3′) primer pair (7,8). Using BLAST version 2.2.9 software (www. ncbi.nlm.nig.gov/BLAST), we determined that this sequence showed 98% similarity with the 16S rDNA sequence of S. mucosissima (GenBank accession no. AM229669). A phylogenetic neighbor-joining tree resulting from comparison of sequences of the 16S rDNA genes of Sphingomonas spp. was made with the MEGA 3.1 software (www.megasoftware.net). This analysis confi rmed that the isolate belonged to S. mucosissima. Initial treatment of intravenous administration of ceftriaxone was begun. The fever resolved after 1 day and the patient’s condition improved. Treatment was stopped after 5 days, and the patient remained apyretic. Two S. mucosissima isolates were recovered from 2 different blood-culture samples drawn 24 hours apart, which suggests that S. mucosissima was not just a transient organism but indeed was responsible for the patient’s septicemia. Phenotypic identifi cation of the gram-negative bacilli failed because the defi nite bacterial species S. mucosissima was not included in the API database (http://industry.biomerieux-usa.com/ industry/food/api/apiweb.htm) used for the phenotypic identifi cation. However, the isolates’ biochemical characteristics were consistent with those previously reported for S. mucosissima (5) (Table). Final identifi cation was achieved by comparing the almost complete 16S rDNA sequence with homologous sequences deposited in GenBank.